Time-Resolved Fluorescence Anisotropy and Molecular Dynamics Analysis of a Novel GFP Homo-FRET Dimer
Yurema Teijeiro-Gonzalez, Alessandro Crnjar, Andrew J. Beavil, Rebecca L. Beavil, Jakub Nedbal, Alix Le Marois, Carla Molteni, Klaus Suhling
Abstract
〉 = 0.17 ± 0.16 and 〈|κ|〉 = 0.35 ± 0.20, contextualized within a static framework in which the linker hinders the free rotation of the fluorophores and excludes certain configurations. The combination of time- and polarization-resolved fluorescence spectroscopy with molecular dynamics simulations is shown to be a powerful tool for the study and interpretation of homo-FRET.
Topics & Concepts
Förster resonance energy transferFluorescence anisotropyDimerFluorescenceGreen fluorescent proteinAnisotropyBiophysicsMolecular dynamicsChemistryPhysicsBiologyComputational chemistryGeneOpticsBiochemistryOrganic chemistryLuminescence and Fluorescent MaterialsOrganic Electronics and PhotovoltaicsGaN-based semiconductor devices and materials