A Conundrum of r-Protein Stability: Unbalanced Stoichiometry of r-Proteins during Stationary Phase in Escherichia coli
Kaspar Reier, Petri‐Jaan Lahtvee, Aivar Liiv, Jaanus Rèmme
Abstract
Ribosome degradation has been extensively described from the viewpoint of its main component, rRNA. Here, we aim to complement our knowledge by quantitatively analyzing r-protein degradation and stability both in the ribosomes and in the whole-cell proteome during stationary phase in E. coli. r-proteins are considered to be very stable in the proteome. Here, we show that a specific set of r-proteins are rapidly degraded after release from the rRNA. The degradation of r-proteins is an intriguing new aspect of r-protein metabolism in bacteria.
Topics & Concepts
RibosomeProteomeEscherichia coliRibosomal proteinChemistryComplement (music)Stationary phaseRibosomal RNAProtein stabilityDegradation (telecommunications)Computational biologyBiochemistryBiologyRNAComputer scienceMutantChromatographyGeneComplementationTelecommunicationsRNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyEnzyme Structure and Function