Comprehensive flow cytometric reference intervals of leukocyte subsets from six study centers across Europe
Astrid Oras, Bibiana Quirant‐Sánchez, Dušan Popadić, Sarah Thunberg, Ola Winqvist, Susanne Heck, M Cwikowski, Dagmar Riemann, Barbara Seliger, Eva Martínez‐Cáceres, Raivo Uibo, Thomas Giese
Abstract
Summary A group of European FOCIS Centers of Excellence adapted panels of the Human Immunophenotyping Consortium (HIPC) for whole blood analysis. Using four core panels [T/regulatory T cell/B/natural killer (T/Treg/B/NK) and myeloid cells] the main leukocyte populations were analyzed in a clinical–diagnostic setting in a harmonized manner across different platforms. As a first step, the consortium presents here the absolute and relative frequencies of the leukocyte subpopulations in the peripheral blood of more than 300 healthy volunteers across six different European centers.