Benchmarking classification abilities of novel optical photothermal IR spectroscopy at the single-cell level with bulk FTIR measurements
Paul I. C. Richardson, Malcolm J. Horsburgh, Royston Goodacre
Abstract
strains as a model and FTIR as a benchmark, we examined O-PTIR's ability to discriminate single-cell samples at the intergenetic, interspecific and intraspecific levels. When combined with chemometric analysis, we showed that O-PTIR is capable of discriminating different between genera, species and strains within species to a degree comparable with FTIR. Furthermore, small variations in the amide bands associated with differences in the protein structure can still be seen in spite of smaller sample sizes. This demonstrates the potential of O-PTIR for single-cell bacterial analysis and classification.
Topics & Concepts
Photothermal therapyBenchmarkingFourier transform infrared spectroscopySpectroscopyMaterials scienceAnalytical Chemistry (journal)Infrared spectroscopyChemistryNanotechnologyOpticsChromatographyPhysicsOrganic chemistryMarketingBusinessQuantum mechanicsSpectroscopy Techniques in Biomedical and Chemical ResearchBiosensors and Analytical DetectionListeria monocytogenes in Food Safety