Supramolecular methods: the 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) transport assay
Alexander M. Gilchrist, Patrick Wang, Israel Carreira‐Barral, Daniel Alonso-Carrillo, Xin Wu, Roberto Quesada, Philip A. Gale
Abstract
The liposomal membrane transport assay, which uses 8-hydroxypyrene-1,3,6-trisulfonic acid to monitor the internal pH of the vesicles (the HPTS assay), is a widely used technique for analysing the activity of anionophore-facilitated transport across a phospholipid membrane. This paper describes the stepwise technique to conduct this transport assay, detailing both the perks and pitfalls of using this method to determine the activity of an anionophore and the transport mechanism.
Topics & Concepts
ChemistryVesicleSupramolecular chemistryMembranePhospholipidMembrane transportLiposomeChromatographyBiophysicsBiochemistryOrganic chemistryMoleculeBiologyLipid Membrane Structure and BehaviorMolecular Sensors and Ion DetectionElectrochemical Analysis and Applications