Cytosolic peptides encoding CaV1 C-termini downregulate the calcium channel activity-neuritogenesis coupling
Yaxiong Yang, Zhen Yu, Jinli Geng, Min Liu, Nan Liu, Ping Li, Weili Hong, Shuhua Yue, He Jiang, Haiyan Ge, Qian Feng, Wei Xiong, Ping Wang, Sen Song, Xiaomei Li, Yubo Fan, Xiaodong Liu
Abstract
Abstract L-type Ca 2+ (Ca V 1) channels transduce channel activities into nuclear signals critical to neuritogenesis. Also, standalone peptides encoded by Ca V 1 DCT (distal carboxyl-terminus) act as nuclear transcription factors reportedly promoting neuritogenesis. Here, by focusing on exemplary Ca V 1.3 and cortical neurons under basal conditions, we discover that cytosolic DCT peptides downregulate neurite outgrowth by the interactions with Ca V 1’s apo-calmodulin binding motif. Distinct from nuclear DCT, various cytosolic peptides exert a gradient of inhibitory effects on Ca 2+ influx via Ca V 1 channels and neurite extension and arborization, and also the intermediate events including CREB activation and c-Fos expression. The inhibition efficacies of DCT are quantitatively correlated with its binding affinities. Meanwhile, cytosolic inhibition tends to facilitate neuritogenesis indirectly by favoring Ca 2+ -sensitive nuclear retention of DCT. In summary, DCT peptides as a class of Ca V 1 inhibitors specifically regulate the channel activity-neuritogenesis coupling in a variant-, affinity-, and localization-dependent manner.