Fluorescence resonance energy transfer using DNA-templated copper nanoparticles for ratiometric detection of microRNAs
Seokjoon Kim, Ki Soo Park
Abstract
We examined the effectiveness of a ratiometric method using DNA-templated copper nanoparticles, which can function as a probe for fluorescence resonance energy transfer. This method in combination with PCR successfully detected the target microRNA, which corresponded well with the results obtained by quantitative reverse transcription PCR.
Topics & Concepts
Förster resonance energy transferFluorescenceNanoparticleCopperEnergy transferChemistryDNAmicroRNAResonance (particle physics)PhotochemistryNanotechnologyMaterials scienceChemical physicsBiochemistryAtomic physicsOrganic chemistryGenePhysicsQuantum mechanicsAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryMicroRNA in disease regulation