GCN4 Regulates Secondary Metabolism through Activation of Antioxidant Gene Expression under Nitrogen Limitation Conditions in Ganoderma lucidum
Lingdan Lian, Lingshuai Wang, Shuqi Song, Jing Zhu, Rui Liu, Liang Shi, Ang Ren, Mingwen Zhao
Abstract
Nitrogen has been widely reported to regulate secondary metabolism in fungi. Our study assessed the specific nitrogen regulatory mechanisms in Ganoderma lucidum. We found that GCN4 directly interacts with the ROS signaling pathway to negatively regulate GA biosynthesis under nitrogen-limiting conditions. Our research highlights a novel insight that GCN4, the nitrogen utilization regulator, participates in secondary metabolism through ROS signal regulation. In addition, this also provides a theoretical foundation for exploring the regulation of other physiological processes by GCN4 through ROS in fungi.
Topics & Concepts
Secondary metabolismReactive nitrogen speciesTranscription factorBiochemistryReactive oxygen speciesGlutathioneBiosynthesisSignal transductionBiologyCell biologyTranscriptional regulationChemistryMetabolic pathwayMetabolismGeneEnzymeFungal Biology and ApplicationsFungal and yeast genetics researchPlant biochemistry and biosynthesis