Janthinobacterium aestuarii sp. nov., a novel violacein-producing bacterium isolated from the Tidal Basin in Washington, D.C., USA
Christina Pavloudi, Maximilian D. Shlafstein, Yesmarie T. De La Flor, Emma M. Smith, Ana Peñuela, Erin Papke, Lausanne Oliver, Kaitlynn Slattery, Guinevere Lissner, Lívia C. Coelho, David K. Oline, Patrick Videau, Wendy K. Strangman, Blake Ushijima, Jimmy H. Saw
Abstract
Strain TB1-E2-13 T was isolated from water collected from the Tidal Basin in Washington, D.C., USA, due to the bright purple colour of its colonies, and was taxonomically evaluated with a polyphasic approach. Comparison of a partial 16S rRNA gene sequence found that strain TB1-E2-13 T was most similar to species in the Janthinobacterium genus. For more precise taxonomic inference, a phylogenomic analysis was conducted and indicated that strain TB1-E2-13 T was most closely related to Janthinobacterium lividum , ‘ Janthinobacterium kumbetense ’, Janthinobacterium rivuli and Janthinobacterium violaceinigrum . Analyses of genomic indices found that pairwise comparisons between strain TB1-E2-13 T and other members of the Janthinobacterium genus returned values below the threshold of species novelty. Based on a polyphasic characterization and identifying differences in genomic and taxonomic data, strain TB1-E2-13 T represents a novel species, for which the name Janthinobacterium aestuarii sp. nov. is proposed. The type strain is TB1-E2-13 T (=ATCC TSD-339 T =JCM 36076 T ).