SYBR Green I promotes melamine binding to poly-thymine DNA and FRET-based ratiometric sensing
Fan He, Yu‐Dong Shen, Juewen Liu
Abstract
can be masked by adding EDTA. Detection of melamine in milk was achieved with recovery rates from 87.0 to 101.7%. This study has provided both basic biochemical insights and a ratiometric sensor for highly sensitive and robust detection of melamine.
Topics & Concepts
SYBR Green IFörster resonance energy transferMelamineChemistryDNABiosensorThymineIntramolecular forceFluorescenceBiophysicsBiochemistryReal-time polymerase chain reactionStereochemistryBiologyGenePhysicsOrganic chemistryQuantum mechanicsAdvanced biosensing and bioanalysis techniquesMelamine detection and toxicityBiosensors and Analytical Detection