Detection of Water Molecules on the Radical Transfer Pathway of Ribonucleotide Reductase by <sup>17</sup>O Electron–Nuclear Double Resonance Spectroscopy
Fabian Hecker, JoAnne Stubbe, Marina Bennati
Abstract
O distances in the range 2.8-3.1 Å. The availability of this structural information will allow for quantitative models of PCET in this prototype enzyme. The results also provide a spectroscopic signature for water H-bonded to a tyrosyl radical.
Topics & Concepts
ChemistryRibonucleotide reductaseMoleculeElectron transferSpectroscopyResonance (particle physics)Nuclear magnetic resonance spectroscopyPhotochemistryElectron nuclear double resonanceElectron paramagnetic resonance spectroscopyNuclear magnetic resonanceElectron paramagnetic resonanceStereochemistryAtomic physicsBiochemistryOrganic chemistryGeneProtein subunitQuantum mechanicsPhysicsElectron Spin Resonance StudiesPhotosynthetic Processes and MechanismsElectrochemical Analysis and Applications