DNA-Interactive and Damage Study with meso-Tetra(2-thienyl)porphyrins Coordinated with Polypyridyl Pd(II) and Pt(II) Complexes
Bernardo A. Iglesias, Níckolas Pippi Peranzoni, Sophia Iwersen Faria, Luana Belo Trentin, André Passáglia Schuch, Otávio Augusto Chaves, Renan Ribeiro Bertoloni, Sofia Nikolaou, Kléber T. de Oliveira
Abstract
We report the DNA-binding properties of three porphyrins with peripheral thienyl substituents (TThPor, PdTThPor and PtTThPor). The binding capacity of each porphyrin with DNA was determined by UV-Vis and steady-state fluorescence emission spectroscopy combined with molecular docking calculations. The results suggest that the interaction of these compounds probably occurs via secondary interactions via external grooves (minor grooves) around the DNA macromolecule. Moreover, porphyrins containing peripheral Pd(II) or Pt(II) complexes (PdTThPor and PtTThPor) were able to promote photo-damage in the DNA.