Inducible CRISPR/Cas9 Allows for Multiplexed and Rapidly Segregated Single-Target Genome Editing in <i>Synechocystis</i> Sp. PCC 6803
Ivana Cengic, Inés C. Cañadas, Nigel P. Minton, Elton P. Hudson
Abstract
) was added to the CRISPR/Cas9 vector. This inducible system allowed for curing of the vector in 25-75% of screened colonies, enabling edited mutants to become markerless.
Topics & Concepts
CRISPRGenome editingComputational biologyCas9GenomeBiologyGuide RNASynechocystisGeneticsSynthetic biologyCyanobacteriaGeneBacteriaCRISPR and Genetic EngineeringEvolution and Genetic DynamicsGenetics, Aging, and Longevity in Model Organisms