Enhancement of intestinal calcium transport by short-chain fatty acids: roles of Na<sup>+</sup>/H<sup>+</sup> exchanger 3 and transient receptor potential vanilloid subfamily 6
Nithipak Thammayon, Kannikar Wongdee, Jarinthorn Teerapornpuntakit, Jiraporn Panmanee, Krittikan Chanpaisaeng, Netnapa Charoensetakul, Nitipol Srimongkolpithak, Panan Suntornsaratoon, Narattaphol Charoenphandhu
Abstract
Organic molecules in the intestinal lumen, e.g., glucose and short-chain fatty acids (SCFAs), the latter of which are normally produced by microfloral fermentation, can stimulate calcium absorption dependent on transient receptor potential vanilloid subfamily 6 (TRPV6) and Na + /H + exchanger 3 (NHE3). A selective TRPV6 inhibitor synthesized and demonstrated by in silico docking and molecular dynamics to specifically bind to the pore domain of TRPV6 was used to confirm a significant contribution of this channel. Our findings corroborate physiological significance of nutrients and SCFAs in enhancing calcium absorption.