The Parallel Reaction Monitoring-Parallel Accumulation–Serial Fragmentation (prm-PASEF) Approach for Multiplexed Absolute Quantitation of Proteins in Human Plasma
Alexander Brzhozovskiy, А. С. Кононихин, Anna E. Bugrova, Kovalev Gi, Pierre-Olivier Schmit, Gary Kruppa, Е. Н. Николаев, Christoph H. Borchers
Abstract
= 0.97) with a slope of close to 1 (0.99), demonstrating that prm-PASEF is well suited for "absolute" quantitative proteomics.
Topics & Concepts
ChemistryMass spectrometryQuantitative proteomicsProteomicsFragmentation (computing)ChromatographySelected reaction monitoringProteomeLabel-free quantificationPeptideTandem mass spectrometryBlood proteinsAnalytical Chemistry (journal)BiochemistryComputer scienceGeneOperating systemAdvanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsMetabolomics and Mass Spectrometry Studies