Synthesis and biological evaluation of chromone-thiazolidine-2,4-dione derivatives as potential α-glucosidase inhibitors
Yingying Zheng, Mengyu Li, Simin Wu, Li Lü, Zhuang Xiong, Xuetao Xu, Kun Zhang, Yi Wen
Abstract
A series of chromone-thiazolidine-2,4-dione derivatives (e1∼28) were synthesized and screened for their α-glucosidase inhibitory. All synthetic derivatives presented excellent α-glucosidase inhibitory with IC50 values ranging from 2.40 ± 0.11 to 5.66 ± 0.15 μM, comparing to positive control acarbose (IC50 value: 640.57 ± 5.13 μM). Among them, compound e28 displayed the strongest α-glucosidase inhibitory (IC50 value: 2.40 ± 0.11), ∼ 267 times stronger than positive control acarbose. Kinetic studies revealed that compound e28 was a reversible non-competitive inhibitor. CD spectra and 3D fluorescence spectra results explained that compound e28 changed the conformational changes of α-glucosidase. Molecular docking simulated the binding between compound e28 and α-glucosidase. In vitro cytotoxicity assay ascertained the good security of e28.