DNase-mediated eDNA removal enhances D-LL-31 activity against biofilms of bacteria isolated from chronic rhinosinusitis patients
Saharut Wongkaewkhiaw, Sakawrat Kanthawong, Jan G. M. Bolscher, Kamran Nazmi, Suwimol Taweechaisupapong, Bastiaan P. Krom
Abstract
value with exogenous DNA resulted in reduced antimicrobial activity. DNase I alone did not show antimicrobial activity against the isolates tested but caused dispersal of an established biofilm. Hence, the presence of eDNA in the biofilm matrix reduced AMP-mediated killing. These results suggest that combination therapy with proteolysis resistant AMP D-LL-31 and DNase could be considered for effective treatment of CRS.
Topics & Concepts
BiofilmMicrobiologyChronic rhinosinusitisBacteriaBiologyChemistryImmunologyGeneticsBacterial biofilms and quorum sensingSinusitis and nasal conditionsAntimicrobial agents and applications