Moonlighting Function of Phytochelatin Synthase1 in Extracellular Defense against Fungal Pathogens
Kian Hématy, Melisa Lim, Candice Cherk, Mariola Piślewska‐Bednarek, Clara Sánchez‐Rodríguez, Mónica Stein, René Fuchs, Christine Klapprodt, Volker Lipka, Antonio Molina, Erwin Grill, Paul Schulze‐Lefert, Paweł Bednarek, Shauna Somerville
Abstract
mutant plants exhibit similar metabolic defects in the accumulation of pathogen-inducible indole glucosinolate-derived compounds, suggesting that PEN2 and PCS1 act in the same metabolic pathway. The function of PCS1 in this pathway is independent of phytochelatin synthesis and deglycination of glutathione conjugates, as catalytic-site mutants of PCS1 are still functional in indole glucosinolate metabolism. In uncovering a peptidase-independent function for PCS1, we reveal this enzyme to be a moonlighting protein important for plant responses to both biotic and abiotic stresses.