Derivatizing Nile Red fluorophores to quantify the heterogeneous polarity upon protein aggregation in the cell
Rui Sun, Wang Wan, Wenhan Jin, Yulong Bai, Qiuxuan Xia, Mengdie Wang, Yanan Huang, Lianggang Zeng, Jialu Sun, Congcong Peng, Biao Jing, Yu Liu
Abstract
Protein aggregation in the cell is often manifested by the formation of subcellular punctate structures. Herein, we modulated the solvatochromism and solubility of Nile Red fluorophore derivatives to quantitatively study the polarity inside pathogenic protein aggregates, revealing structure- and protein-dependent polarity heterogeneity.
Topics & Concepts
Nile redPolarity (international relations)FluorophoreSolvatochromismProtein aggregationChemistryBiophysicsSolubilityFluorescenceCellBiochemistryOrganic chemistryBiologyMoleculeOpticsPhysicsProtein Structure and DynamicsLipid Membrane Structure and BehaviorHemoglobin structure and function