Turn-on Fluorescent Biosensors for Imaging Hypoxia-like Conditions in Living Cells
Santiago Guisán‐Ceinos, Alexandra R. Rivero, Fernando Romeo‐Gella, Silvia Simón‐Fuente, Silvia Gómez-Pastor, Natalia Calvo, Alejandro H. Orrego, José M. Guisán, Inés Corral, Francisco Sanz-Rodrı́guez, M. Ribagorda
Abstract
potential energy surface following the reduction of the azo moiety and rationalize the fluorescent quenching event through the mapping of an unprecedented pathway. Fluorescent microscopy experiments show that these azos can be used to visualize hypoxia-like conditions within living cells.
Topics & Concepts
BODIPYChemistryFluorescenceBiosensorMoietyCombinatorial chemistryConjugated systemQuenching (fluorescence)Fluorescence microscopePhotochemistryBiophysicsNanotechnologyBiochemistryOrganic chemistryPolymerMaterials sciencePhysicsQuantum mechanicsBiologyLuminescence and Fluorescent MaterialsPhotochromic and Fluorescence ChemistryNanoplatforms for cancer theranostics