Molecular Surveillance of Malaria Using the PF AmpliSeq Custom Assay for Plasmodium falciparum Parasites from Dried Blood Spot DNA Isolates from Peru
Johanna Helena Kattenberg, Norbert van Dijk, Carlos Fernández-Miñope, Pieter Guetens, Mathijs Mutsaers, Dionicia Gamboa, Anna Rosanas‐Urgell
Abstract
), using Illumina short-read sequencing technology. The assay applies a multiplex PCR approach to amplify all genomic regions of interest in a rapid and easily standardizable procedure and allows simultaneous amplification of a high number of targets at once, therefore having great potential for implementation into routine surveillance practice by NMCPs. The assay can be performed on routinely collected filter paper blood spots and can be easily adapted to different regions to investigate either regional trends or in-country epidemiological changes.
Topics & Concepts
MalariaPlasmodium falciparumVirologyDried blood spotBiologyGeneticsImmunologyMalaria Research and ControlMosquito-borne diseases and controlVector-borne infectious diseases