Assessment of human plasma and urine sample preparation for reproducible and high-throughput UHPLC-MS clinical metabolic phenotyping
Andrew D. Southam, Liam D. Haglington, Lukáš Najdekr, Andris Jankevics, Ralf J. M. Weber, Warwick B. Dunn
Abstract
UHPLC-MS plasma lipidomics, monophasic 100% isopropanol had the highest detection response of all annotated lipid classes with high reproducibility. Increasing monophasic incubation time and temperature had little benefit on metabolite and lipid yield and reproducibility for all methods.
Topics & Concepts
UrineSample preparationHuman plasmaChromatographyReproducibilityMetaboliteUrine sampleChemistryBiochemistryMetabolomics and Mass Spectrometry StudiesGut microbiota and healthAnalytical Chemistry and Chromatography