Bacterial H-NS contacts DNA at the same irregularly spaced sites in both bridged and hemi-sequestered linear filaments
Beth A. Shen, Christine M. Hustmyer, Daniel Roston, Michael B. Wolfe, Robert Landick
Abstract
-EDTA) tethered to the DBDs (TEN-map). We find that H-NS DBDs contact DNA at indistinguishable locations in bridged or linear filaments and that the DBDs vary in orientation and position with ∼10-bp average spacing. Our results support a hemi-sequestration model of linear-to-bridged H-NS switching. Linear filaments able to inhibit only transcription initiation switch to bridged filaments able to inhibit both initiation and elongation using the same irregularly spaced DNA contacts.
Topics & Concepts
DNANucleoproteinBiophysicsChemistryTranscription bubbleChromatinTranscription (linguistics)CrystallographyRNA polymeraseRNAMolecular biologyBiologyGeneBiochemistryLinguisticsPhilosophyBacterial Genetics and BiotechnologyRNA and protein synthesis mechanismsBacteriophages and microbial interactions