Unveiling autophagy and aging through time-resolved imaging of lysosomal polarity with a delayed fluorescent emitter
Subhadeep Das, Abhilasha Batra, Subhankar Kundu, Rati Sharma, Abhijit Patra
Abstract
A TADF-emitter was employed to unveil the lysosomal polarity variations during interorganelle interactions and was demonstrated as an indicator to probe complex biological processes like aging through intracellular lifetime imaging.
Topics & Concepts
Multicellular organismPolarity (international relations)LysosomeAutophagyLive cell imagingMitophagyCell biologyOrganelleFluorescence-lifetime imaging microscopyFluorescenceBiophysicsCaenorhabditis elegansBiologyChemistryCellBiochemistryGeneQuantum mechanicsEnzymePhysicsApoptosisCalcium signaling and nucleotide metabolismAutophagy in Disease and TherapyCellular transport and secretion