Quantitative tracking of endoplasmic reticulum viscosity during ferroptosis by an iridium complex <i>via</i> TPPLM
Hao Liang, Yanmei Zhong, Cai-Ping Tan, Zong‐Wan Mao
Abstract
Herein, we report a neutral iridium complex, [Ir(4-(2-pyridinyl)benzaldehyde)2(acetylacetone)] (Ir-ER), with viscosity-responsive phosphorescent emission intensity and lifetime. Quantitative measurement by two-photon phosphorescent lifetime imaging shows that the viscosity of ER increases significantly in the process of erastin-induced ferroptosis. Our work provides an effective strategy for quantitative measurement of the micro-environmental alternations of subcellular organelles during a specific cell death process.
Topics & Concepts
Endoplasmic reticulumTracking (education)IridiumViscosityChemistryPhosphorescenceCell biologyBiophysicsMaterials scienceBiochemistryBiologyPhysicsCatalysisOpticsPsychologyComposite materialPedagogyFluorescenceExtracellular vesicles in diseaseFerroptosis and cancer prognosisAdvanced biosensing and bioanalysis techniques