Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells
Giuliano Giuseppe Stirparo, Austin Smith, Ge Guo
Abstract
Previous analysis of RNA sequencing (RNA-seq) data from human naive pluripotent stem cells reported multiple point "mutations" in cancer-related genes and implicated selective culture conditions. We observed, however, that those mutations were only present in co-cultures with mouse feeder cells. Inspection of reads containing the polymorphisms revealed complete identity to the mouse reference genome. After we filtered reads to remove sequences of mouse origin, the actual incidence of oncogenic polymorphisms arising in naive pluripotent stem cells is close to zero.
Topics & Concepts
BiologyInduced pluripotent stem cellGeneticsGeneCancerStem cellPoint mutationMutationGenomeComputational biologyCancer researchMolecular biologyEmbryonic stem cellPluripotent Stem Cells ResearchCRISPR and Genetic EngineeringCancer Genomics and Diagnostics