First Report of Resistance-Breaking Variants of Tomato Spotted Wilt Virus (TSWV) Infecting Tomatoes with the <i>Sw-5</i> Resistance Gene in North Carolina
Kirsten A. Lahre, Reza Shekasteband, Inga Meadows, Anna E. Whitfield, Dorith Rotenberg
Abstract
, the western flower thrips. Symptomatic leaf tissue obtained from these inoculation assays tested positive for TSWV by DAS-ELISA (AgDia, Elkhart, IN) and RT-PCR with NSm primers, providing definitive evidence of the occurrence of RB-TSWV at both farms, and subsequent sequencing confirmed the C118Y and D122G substitutions. This report warrants further investigation of the putative origins, prevalence and epidemiological implications of RB-TSWV variants in NC tomato production, and the development of new sources of resistance to TSWV.
Topics & Concepts
Agricultural experiment stationEntomologyBiologyPlant diseaseResistance (ecology)Library scienceEcologyBiotechnologyAgricultureComputer sciencePlant Virus Research StudiesPlant and Fungal Interactions ResearchPlant Pathogenic Bacteria Studies