Identification of an Emergent Pathogen, <i>Bartonella vinsonii</i>, Using Next-Generation Sequencing in a Patient With Culture-Negative Endocarditis
Rachel Downey, Susan Russo, Sarmistha B. Hauger, Donald Murphey, Grace E. Marx, Thanhthao Huynh, Amy M. Denison, Rachel A Quirt, Ann Bailey, Marisol Fernández
Abstract
Diagnosis and treatment of culture negative endocarditis remains a challenge. This report describes a rare cause of endocarditis in humans, Bartonella vinsonii, identified through next generation sequencing of plasma microbial cell-free DNA with confirmation of cardiac valve tissue infection through immunohistochemical staining and polymerase chain reaction.
Topics & Concepts
EndocarditisMedicineIdentification (biology)PathogenBartonellaDNA sequencingPathogenic organismComputational biologyMicrobiologyVirologyGeneticsImmunologyBiologyGeneInternal medicineBotanyBartonella species infections researchInfective Endocarditis Diagnosis and ManagementStreptococcal Infections and Treatments