<i>Aspergillus fumigatus</i> Hsp90 interacts with the main components of the cell wall integrity pathway and cooperates in heat shock and cell wall stress adaptation
Marina Campos Rocha, Karine Minari, João Henrique Tadini Marilhano Fabri, Joshua D. Kerkaert, Lisandra M. Gava, Anderson Ferreira da Cunha, Robert A. Cramer, Júlio C. Borges, Iran Malavazi
Abstract
also abolishes MpkA activation in the short-term response to HS. Biochemical and biophysical analyses indicated that Hsp90 is a dimeric functional ATPase, which has a higher affinity for ADP than ATP and prevents MpkA aggregation in vitro. Our data suggest that the CWIP is constitutively required for A. fumigatus to cope with the temperature increase found in the mammalian lung environment, emphasising the importance of this pathway in supporting thermotolerance and cell wall integrity.
Topics & Concepts
Aspergillus fumigatusBiologyHsp90Heat shock proteinCell biologyHsp90 inhibitorMicrobiologyCell wallChaperone (clinical)BiochemistryGeneMedicinePathologyHeat shock proteins researchthermodynamics and calorimetric analysesComputational Drug Discovery Methods