Quantified Detection of Treponema pallidum DNA by PCR Assays in Urine and Plasma of Syphilis Patients
Cuini Wang, Xin Zheng, Zhifang Guan, Danyang Zou, Xin Gu, Haikong Lu, Mei Shi, Pingyu Zhou
Abstract
T. pallidum can invade many organs, and T. pallidum DNA can be detected in various tissues and fluids. The results reported here demonstrated that T. pallidum DNA could be detected in urine in patients at all stages of syphilis. The detection rate and loads of T. pallidum DNA in urine sediment were significantly higher than those in urine supernatant. Urine is abundant, and its collection is noninvasive and convenient; therefore, urine is an ideal sample for acquiring a large amount of T. pallidum DNA, which can be supplement samples for the detection of molecular typing of T. pallidum.
Topics & Concepts
TreponemaUrineSyphilisNeurosyphilisDNAMedicineBiologyVirologyInternal medicineGeneticsHuman immunodeficiency virus (HIV)Syphilis Diagnosis and TreatmentReproductive tract infections researchSex work and related issues