A multicomponent reaction platform towards multimodal near-infrared BODIPY dyes for STED and fluorescence lifetime imaging
Antonio Fernández, Nicola Kielland, Ashraff Makda, Neil O. Carragher, M. Carmen González-García, Laura Espinar‐Barranco, Juan A. González‐Vera, Ángel Orte, Rodolfo Lavilla, Marc Vendrell
Abstract
We report a platform combining multicomponent reaction synthesis and automated cell-based screening to develop biocompatible NIR-BODIPY fluorophores. From a library of over 60 fluorophores, we optimised compound NIRBD-62c as a multimodal probe with suitable properties for STED super-resolution and fluorescence lifetime imaging. Furthermore, we employed NIRBD-62c for imaging trafficking inside cells and to examine how pharmacological inhibitors can alter the vesicular traffic between intracellular compartments and the plasma membrane.
Topics & Concepts
STED microscopyBODIPYFluorescenceFluorescence-lifetime imaging microscopyLive cell imagingNanotechnologyChemistryBiophysicsSuperresolutionMaterials scienceCellComputer scienceOpticsBiochemistryArtificial intelligencePhysicsImage (mathematics)BiologyNanoplatforms for cancer theranosticsClick Chemistry and ApplicationsLuminescence and Fluorescent Materials