Diagnosis of methylglyoxal in blood by using far-infrared spectroscopy and o-phenylenediamine derivation
Xu Wu, Yang Dai, Liping Wang, Yan Peng, Lin Lü, Yiming Zhu, Yijue Shi, Songlin Zhuang
Abstract
Methyglyoxal (MGO) is an important pathological factor for diabetic cardiovascular complications. Conventional methods for MGO detection in biological samples, such as high performance liquid chromatography (HPLC)-UV spectrometry, LC-fluorescence spectrometry, and HPLC-mass spectrometry, are time-consuming, high-cost, and complicated. Here, we present a method for MGO quantitative detection based on far-IR spectral analyses. Our method uses o-phenylenediamine (OPD) to produce a chemical reaction with MGO, which results in multiple fingerprint feature changes associated with the molar ratio of MGO and OPD. We use the linear relationship between MGO concentration and peak intensity of the reaction product to quantitatively determine MGO concentration. The corresponding linear detectable range is 5∼2500 nmol/mL nmol per mL with a correlation coefficient of 0.999. This quantitative method is also tested by blood samples with adjusted MGO concentrations, and shows 95% accuracy with only 30s testing time. Our method provides a fast, simple and economical approach to determining MGO concentration in blood.