Update of the Pyrrolysyl-tRNA Synthetase/tRNA <sup>Pyl</sup> Pair and Derivatives for Genetic Code Expansion
Xuemei Gong, Haolin Zhang, Yue Shen, Xian Fu
Abstract
The cotranslational incorporation of pyrrolysine (Pyl), the 22nd proteinogenic amino acid, into proteins in response to the UAG stop codon represents an outstanding example of natural genetic code expansion. Genetic encoding of Pyl is conducted by the pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNA, tRNA Pyl .
Topics & Concepts
BiologyGenetic codeTransfer RNAGeneticsBiochemistryDNARNAGeneRNA and protein synthesis mechanismsRNA modifications and cancerGenomics and Phylogenetic Studies