Real-time monitoring of ONOO⁻ in cerebral ischemia-reperfusion injury mouse models using a hydrazine-based NIR fluorescent probe
Jinting Shang, Yan Yang, Yaojian Sun, Wanxia Gao, Kang Ma, Wang Chen, Xin Yu, Liping Li, Jiang Zheng, Na Zhao, Xiji Shu, Yibin Zhang
Abstract
Accurate and selective techniques for visualizing endogenous peroxynitrite (ONOO − ) in cerebral ischemia-reperfusion injury (CIRI) models are essential for understanding its complex pathological processes. Here, we introduced a longwave fluorescent probe TJO for detecting ONOO − rapidly and sensitively, with a low detection limit of 91 nM. Furthermore, TJO exhibits excellent fluorescence imaging capabilities, enabling detailed visualization of ONOO⁻ in CIRI mice model. This highlights its potential for real-time monitoring of ONOO⁻-related pathological conditions. • TJO can selectively and quantitatively detect ONOO − in the near-infrared region. • The reaction mechanism is elucidated through theoretical calculations and LC-MS analysis. • TJO visualized ONOO − in living CIRI model mice, highlighting its potential for real-time tracking of related ONOO − pathology.