3D Printing of Bone‐Mimetic Scaffold Composed of Gelatin/β‐Tri‐Calcium Phosphate for Bone Tissue Engineering
Jae Eun Jeong, Shin Young Park, Shin Young Park, Ji Youn Shin, Ji Min Seok, June Ho Byun, Se Heang Oh, Wan Doo Kim, Jun Hee Lee, Won Ho Park, Su A Park, Su A Park
Abstract
Abstract 3D printed scaffolds composed of gelatin and β‐tri‐calcium phosphate (β‐TCP) as a biomimetic bone material are fabricated, thereby providing an environment appropriate for bone regeneration. The Ca 2+ in β‐TCP and COO − in gelatin form a stable electrostatic interaction, and the composite scaffold shows suitable rheological properties for bioprinting. The gelatin/β‐TCP scaffold is crosslinked with glutaraldehyde vapor and unreacted aldehyde groups which can cause toxicity to cells is removed by a glycine washing. The stable binding of the hydrogel is revealed as a result of FTIR and degradation rate. It is confirmed that the composite scaffold has compressive strength similar to that of cancellous bone and 60 wt% β‐TCP groups containing 40 wt% gelatin have good cellular activity with preosteoblasts. Also, in the animal experiments, the gelatin/β‐TCP scaffold confirms to induce bone formation without any inflammatory responses. This study suggests that these fabricated scaffolds can serve as a potential bone substitute for bone regeneration.