A Folding-Based Electrochemical Aptasensor for the Single-Step Detection of the SARS-CoV-2 Spike Protein
Federica Curti, Simone Fortunati, Wolfgang Knoll, Marco Giannetto, Roberto Corradini, Alessandro Bertucci, Maria Careri
Abstract
) value of 43 ± 4 nM and a limit of detection of 7 nM. We demonstrate that the target S1 protein can be detected both in a buffer solution and in an artificial viral transport medium widely used for the collection of nasopharyngeal swabs, and that no cross-reactivity is observed in the presence of different, non-target viral proteins. We expect that this SWCNT-SPE-based format of electrochemical aptasensor will prove useful for the detection of other protein targets for which nucleic acid aptamer ligands are made available.
Topics & Concepts
AptamerAmperometryMaterials scienceCombinatorial chemistryDetection limitBiosensorFolding (DSP implementation)ElectrochemistryTarget proteinBiophysicsElectrodeNanotechnologyChemistryBiochemistryChromatographyMolecular biologyBiologyEngineeringPhysical chemistryElectrical engineeringGeneAdvanced biosensing and bioanalysis techniquesSARS-CoV-2 detection and testingBiosensors and Analytical Detection