Generation of Recombinant Antibodies in HEK293F Cells for the Detection of <i>Staphylococcus aureus</i>
Joo-Kyung Kim, Gyumin Lim, Eun-Jung Kim, Wooseong Kim, Chang‐Soo Lee, Byung‐Gee Kim, Hee‐Jin Jeong
Abstract
Staphylococcus aureus is a major resistant pathogen in clinical practice. Due to the increasing number of infections, rapid and sensitive detection of antibiotic-resistant S. aureus as well as antibiotic-sensitive S. aureus is important for the prevention and control of infectious diseases. In this study, we produced recombinant antibodies against S. aureus from mammalian human embryonic kidney 293 Freestyle cells with high yield and purity. These recombinant antibodies showed high binding affinity and low detection limit in both indirect and sandwich enzyme-linked immunosorbent assays for the detection of methicillin-resistant S. aureus and methicillin-sensitive S. aureus. These results suggest that the recombinant antibodies produced herein can be used for the accurate detection of S. aureus with a wild range of applications in medical diagnosis, food safety, and drug discovery.