Microfluidic production of nanogels as alternative triple transfection reagents for the manufacture of adeno-associated virus vectors
Zoe Whiteley, Giulia Massaro, Georgios Gkogkos, Asterios Gavriilidis, Simon N. Waddington, Ahad A. Rahim, Duncan Q.M. Craig
Abstract
Microfluidic produced nanogels provide an alternative to standard transfection reagents for the manufacture of adeno-associated viral vectors (AAVs), with equivelant viral titres demonstrated.
Topics & Concepts
MicrofluidicsTransfectionReagentNanotechnologyMaterials scienceAdeno-associated virusVector (molecular biology)ChemistryBiologyCell cultureRecombinant DNAGeneBiochemistryGeneticsPhysical chemistryVirus-based gene therapy researchRNA Interference and Gene DeliveryViral Infectious Diseases and Gene Expression in Insects