SOI-Nanowire Biosensor for the Detection of Glioma-Associated miRNAs in Plasma
Kristina A. Malsagova, Tatyana O. Pleshakova, Rafael A. Galiullin, Andrey F. Kozlov, Tatyana S. Romanova, Ivan D. Shumov, V. P. Popov, Fedor V. Tikhonenko, Alexander V. Glukhov, Alexander Yu. Smirnov, О А Гаджиева, Boris A. Bashiryan, V. N. Shimansky, Alexander I. Archakov, Yuri D. Ivanov
Abstract
Herein, we report the development of a highly sensitive nanotechnology-based system—silicon-on-insulator nanowire biosensor for the revelation of microRNAs (miRNAs), associated with the development of glioma in the human. In this system, a sensor chip, bearing an array of silicon nanowire structures, is employed. The sensor chip is fabricated using a top-down technology. In our experiments reported herein, we demonstrated the detection of DNA oligonucleotide (oDNA), which represents a synthetic analogue of microRNA-363 associated with the development of glioma. To provide biospecific detection of the target oligonucleotides, the surface of the nanowire structures is modified with oligonucleotide probes; the latter are complementary to the target ones. The concentration limit of the target oligonucleotide detection, attained using our nanowire biosensor, is at the level of DL~10−17 M. The revelation of the elevated level of glioma-associated miRNA in plasma is also demonstrated.