Multiplex PCR Assay for Clade Typing of Salmonella enterica Serovar Enteritidis
Sarah Gallichan, Blanca M. Perez-Sepulveda, Nicholas Feasey, Jay C. D. Hinton, Juno Thomas, Anthony M. Smith
Abstract
Challenges in the diagnosis and treatment of invasive Salmonella Enteritidis bloodstream infections in sub-Saharan Africa are responsible for a case fatality rate of approximately 15%. It is important to identify distinct clades of S. Enteritidis in diagnostic laboratories in the African setting to determine the different health outcomes associated with particular outbreaks. Here, we describe the development of a high-quality molecular classification assay for clade typing of S. Enteritidis that is ideal for use in public health laboratories in resource-limited settings.
Topics & Concepts
CladeSalmonella enteritidisSerotypeSalmonellaBiologySalmonella entericaOutbreakVirologyTypingMicrobiologyPhylogeneticsGeneticsGeneBacteriaSalmonella and Campylobacter epidemiologyViral gastroenteritis research and epidemiologyBacteriophages and microbial interactions