Multiomics assessment of dietary protein titration reveals altered hepatic glucose utilization
Michael R. MacArthur, Sarah J. Mitchell, Katia S. Chadaideh, J. Humberto Treviño-Villarreal, Jonathan Jung, Krystle C. Kalafut, Justin S. Reynolds, Charlotte G. Mann, Kaspar Trocha, Ming Tao, Tay-Zar Aye Cho, Anantawat Koontanatechanon, Vladimir Yeliseyev, Lynn Bry, Alban Longchamp, C. Keith Ozaki, Caroline A. Lewis, Rachel N. Carmody, James R. Mitchell
Abstract
C6 glucose and glycerol tracing reveal increased fractional enrichment in aspartate, serine, and glutamate. Changes remain intact in hepatic ATF4 knockout mice. Together, this demonstrates an ATF4-independent shift in gluconeogenic substrate utilization toward specific AAs, with compensation from glycerol to promote a protein-sparing response.
Topics & Concepts
MetabolomeBiologyGluconeogenesisMetabolismTranscriptomeInternal medicineCarbohydrate metabolismEndocrinologySerineGlucose homeostasisBiochemistryLipid metabolismCitric acid cycleMetaboliteInsulinEnzymeInsulin resistanceGene expressionMedicineGeneDiet and metabolism studiesGut microbiota and healthDietary Effects on Health