Pressure-induced constriction of the middle cerebral artery is abolished in TrpC6 knockout mice
Zoltán Németh, Emily Hildebrandt, Michael J. Ryan, Joey P. Granger, Heather A. Drummond
Abstract
Pressure-induced, but not agonist-induced, vasoconstriction is abolished in the middle cerebral artery (MCA) of TrpC6 null mice. TrpC6 localization in dissociated cerebral vascular smooth muscle cells is primarily cytoplasmic and not associated with the surface membrane where a mechanoelectrical coupler might be expected. These findings suggest that TrpC6 is required for transduction of pressure-induced constriction in the MCA; however, its role as a mechanoelectrical coupler or downstream signal amplifier remains unresolved.
Topics & Concepts
TRPC6VasoconstrictionMiddle cerebral arteryConstrictionKnockout mouseConditional gene knockoutAgonistCerebral arteriesInternal medicineChemistryEndocrinologyCell biologyNeuroscienceAnatomyMedicineBiologyTransient receptor potential channelReceptorIschemiaPhenotypeBiochemistryGeneErythrocyte Function and PathophysiologyIon Channels and ReceptorsIon channel regulation and function