Development of Human Gut Organoids With Resident Tissue Macrophages as a Model of Intestinal Immune Responses
Satoru Tsuruta, Tomoyuki Kawasaki, Masakazu Machida, Ken Iwatsuki, Akihiko Inaba, Shinsuke Shibata, Tomoko Shindo, Kazuhiko Nakabayashi, Kenichi Hakamada, Akihiro Umezawa, Hidenori Akutsu
Abstract
Intestinal macrophages are largely responsible for the innate immune response and also for intestinal homeostasis.1 We have developed novel xenogeneic-free human intestinal organoids (XF-HIOs) that are uniquely structured with an apical-out mucosal epithelium and complex mesenchymal tissue, including smooth muscle and intestinal nerve cells.2,3 To further develop XF-HIOs containing tissue macrophages, we first prepared human-induced pluripotent stem cell (hiPSC)-derived monocyte-like cells (pMCs).
Topics & Concepts
OrganoidImmune systemImmunologyBiologyMicrobiologyMedicineCell biologyNeonatal Respiratory Health ResearchTissue Engineering and Regenerative MedicineDigestive system and related health