Arp2/3 complex and the pentose phosphate pathway regulate late phases of neutrophil swarming
Katharina M. Glaser, Jacob Doon‐Ralls, Nicole Walters, Xilal Y. Rima, Angelika S. Rambold, Eduardo Reátegui, Tim Lämmermann
Abstract
swarming-on-a-chip platform for the use with confocal laser-scanning microscopy to unravel the complexity of single-cell responses during neutrophil crowding. Confocal sectioning allowed the live visualization of subcellular components, including mitochondria, cell membranes, cortical actin, and phagocytic cups, inside neutrophil clusters. Based on this experimental setup, we identify that chemical inhibition of the Arp2/3 complex causes cell death in crowding neutrophils. By visualizing spatiotemporal patterns of reactive oxygen species (ROS) production in developing neutrophil swarms, we further demonstrate a regulatory role of the metabolic pentose phosphate pathway for ROS production and neutrophil cluster growth.