Immuno-chromatic probe based lateral flow assay for point-of-care detection of Japanese encephalitis virus NS1 protein biomarker in clinical samples using a smartphone-based approach
Akanksha Roberts, Drishya Prakashan, Himani Dhanze, Ravi Kumar Gandham, Sonu Gandhi, G. Taru Sharma
Abstract
, which corresponded to the LOD determined by the graphical data obtained from ImageJ software and the Colorimeter smartphone application. Furthermore, the colorimetric based immunosensor showed minimal non-specific detection of other closely related flaviviral NS1 Ags in the spiked serum, provided a rapid result within 10 min, showed storage stability up to a month at 4 °C, successfully detected the JEV NS1 protein in clinically infected pig serum samples, and hence, may be developed into a PoC screening diagnostic kit for JEV.
Topics & Concepts
BiomarkerFlow cytometryPoint-of-care testingColorimeterVirologyJapanese encephalitisVirusEncephalitisMedicineMolecular biologyBiologyPathologyBiochemistryPhysicsQuantum mechanicsMosquito-borne diseases and controlCytomegalovirus and herpesvirus researchViral Infections and Immunology Research