Ubiquitinated ligation protein NEDD4L participates in MiR-30a-5p attenuated atherosclerosis by regulating macrophage polarization and lipid metabolism
Fei Song, Jing-Zhou Li, Yao Wu, Wei‐Yin Wu, Yan Wang, Gang� Li
Abstract
mice and LPS-treated RAW264.7 macrophages by the regulation of Smad-1/2 phosphorylation. MiR-30a-5p reduced lipid uptake in oxidized low-density lipoprotein-treated macrophages by regulating the expression of PPAR-γ, ABCA1, ABCG1, LDLR, and PCSK9. Ubiquitinated ligase NEDD4L was identified as a target of miR-30a-5p. Interestingly, knockdown of NEDD4L decreased the M1/M2 ratio and oxidized low-density lipoprotein uptake in macrophages by inhibiting the ubiquitination of PPAR-γ and phosphorylation of Smad-1/2 and regulating ABCA1, ABCG1, LDLR, and PCSK9. We demonstrated a novel effect and mechanism of miR-30a-5p in atherosclerosis.
Topics & Concepts
ABCG1ChemistryABCA1LipoproteinApolipoprotein BEndocrinologyMacrophage polarizationUbiquitin ligaseInternal medicineUbiquitinMacrophageBiologyCholesterolBiochemistryIn vitroMedicineGeneTransporterMicroRNA in disease regulationCancer-related gene regulationPeroxisome Proliferator-Activated Receptors