Molecular insights into the interaction between human nicotinamide phosphoribosyltransferase and Toll-like receptor 4
Massimiliano Gasparrini, Francesca Mazzola, Massimiliano Cuccioloni, Leonardo Sorci, Valentina Audrito, Federica Zamporlini, Carlo Fortunato, Adolfo Amici, Michele Cianci, Silvia Deaglio, Mauro Angeletti, Nadia Raffaelli
Abstract
values of 0.63 and 0.22 μM, respectively. Mutations also impaired the ability of NAMPT to trigger the NF-κB inflammatory signaling pathway in human cultured macrophages. Finally, the involvement of the two loops in receptor binding was supported by NAMPT-TLR4 docking simulations. This study paves the way for future development of compounds that selectively target eNAMPT/TLR4 signaling in inflammatory disorders.
Topics & Concepts
Nicotinamide phosphoribosyltransferaseNAD+ kinaseEnzymeAlanineNicotinamideTLR4ReceptorChemistryBiochemistryDocking (animal)BiologyCell biologyAmino acidMedicineNursingImmune Response and InflammationInflammasome and immune disordersCytomegalovirus and herpesvirus research