A Disease-Causing Single Amino Acid Deletion in the Coiled-Coil Domain of RAD50 Impairs MRE11 Complex Functions in Yeast and Humans
Marie Chansel-Da Cruz, Marcel Hohl, Ilaria Ceppi, Laëtitia Kermasson, Laurence Maggiorella, Mauro Modesti, Jean‐Pierre de Villartay, Talia İleri, Petr Ćejka, John H.J. Petrini, Patrick Revy
Abstract
-dependent signaling. These findings demonstrate that a minor heptad break in the RAD50 coiled coil suffices to impede MRE11 complex functions in human and yeast. Furthermore, these results emphasize the importance of the RAD50 coiled coil to regulate MRE11-dependent DNA end resection in humans.
Topics & Concepts
Rad50BiologyReplication protein ADNA repairSaccharomyces cerevisiaeCoiled coilDNA damageMutationGeneticsDNACell biologyNucleaseDNA-binding proteinYeastGeneTranscription factorDNA Repair MechanismsGenomics and Chromatin DynamicsPARP inhibition in cancer therapy