Analysis of protein denaturation, aggregation and post-translational modification by agarose native gel electrophoresis
Chiaki Sakuma, Yui Tomioka, Cynthia Li, Takashi Shibata, Masataka Nakagawa, Yasunori Kurosawa, Tsutomu Arakawa, Teruo Akuta
Topics & Concepts
ChemistryAgaroseAgarose gel electrophoresisGel electrophoresisMolecular-weight size markerNative stateBovine serum albuminBiochemistryDenaturation (fissile materials)Gel electrophoresis of proteinsTwo-dimensional gel electrophoresisGel electrophoresis of nucleic acidsChromatographyPolyacrylamide gel electrophoresisMolecular biologyEnzymeProteomicsDNABiologyNuclear chemistryGeneProtein purification and stabilityProtein Structure and DynamicsEnzyme Structure and Function