Tracing Carbon Metabolism with Stable Isotope Metabolomics Reveals the Legacy of Diverse Carbon Sources in Soil
Roland C. Wilhelm, Samuel E. Barnett, Tami L. Swenson, Nicholas D. Youngblut, Chantal Koechli, Benjamin P. Bowen, Trent R. Northen, Daniel H. Buckley
Abstract
C-labeled metabolite profiles corresponded with compositional differences in the metabolically active populations, providing a basis for how microbial community composition correlates with the quality of soil carbon. Our study demonstrates the application of SIP-metabolomics in studying soils and identifies several metabolite markers of growth, activity, and other aspects of microbial function.
Topics & Concepts
MetaboliteMetabolomicsChemistryGlucuronateIsotopes of carbonCarbon fibersUracilStable-isotope probingSoil waterEnvironmental chemistryBiochemistryBiologyBacteriaMicroorganismDNATotal organic carbonChromatographyGeneticsComposite materialEcologyComposite numberMaterials scienceMetabolomics and Mass Spectrometry StudiesMicrobial Community Ecology and PhysiologyIsotope Analysis in Ecology